Lysates were incubated overnight with protein-G dyna beads pre-bound to SMC3(ac) antibody (Nishiyama et al

Lysates were incubated overnight with protein-G dyna beads pre-bound to SMC3(ac) antibody (Nishiyama et al., 2010) or mouse non-immune IgG (SACSC-2025). 2figure product 2source data 1: The Microsoft Excel file lists FRAP measurements used to generate data in Number 2figure product 2. elife-52091-fig2-figsupp2-data1.xlsx (178K) GUID:?7112B48E-DE01-4599-BCA6-6868B095E769 Figure 2figure supplement 3source data 1: The Microsoft Excel file lists FRAP measurements used to generate data in Figure 2figure supplement 3CCF. elife-52091-fig2-figsupp3-data1.xlsx (37K) GUID:?03FE0330-8C00-40E5-8D11-C8175C7391F7 Figure 2figure supplement 3source data 2: The Microsoft Excel file lists FRAP measurements used to generate data in Figure 2figure supplement 3HCL. elife-52091-fig2-figsupp3-data2.xlsx (37K) GUID:?4A77E55B-9CAE-4793-8837-C76EA0E2ACB9 Figure 3source data 1: The Microsoft Excel file lists iFRAP measurements used to AMG 337 generate data in Figure 3B,C,F,G. elife-52091-fig3-data1.xlsx (198K) GUID:?74BD37E2-BF62-4F59-8531-749C74366229 Figure 3source data 2: The Microsoft Excel file lists iFRAP measurements used to generate data in Figure 3ICJ. elife-52091-fig3-data2.xlsx (55K) GUID:?FE3E4969-7CF1-4935-B8A8-E0476CB4E054 Number 3figure product 1source data 1: The Microsoft Excel file lists iFRAP measurements used to generate data in Number 3figure product 1B-E. elife-52091-fig3-figsupp1-data1.xlsx (202K) GUID:?CA6213FF-616C-4606-B465-A5B069CF3183 Figure 3figure supplement 1source data 2: The Microsoft Excel file lists iFRAP measurements used to generate data in Figure 3figure supplement 1G-J. elife-52091-fig3-figsupp1-data2.xlsx (258K) GUID:?0DD03991-6EA9-4E05-8AAA-524085F7EA61 Number 3figure supplement 3source data 1: The Microsoft Excel file lists FRAP measurements used to generate data in Number 3figure supplement 3. elife-52091-fig3-figsupp3-data1.xlsx (88K) GUID:?7530F176-8B59-49D9-A97B-89ECDBA219BA Supplementary file 1: Summary statistics for Hi-C data sets generated with this study. A. Quantity of the library. B. Condition used to generate the library. C. Quantity of the biological replicate. D. Restriction enzyme used to generate the Hi-C library. E. Raw quantity of go through pairs from paired-end sequencing. F. Unique valid mapped go through pairs from HiCUP v0.7.1. G. Quantity of unique valid read pairs that are inter-chromosomal. H. Percentage of unique valid read pairs that are inter-chromosomal. I. Log2 contact enrichment of A-A and B-B contacts for long-range ( 10 Mb) intra-chromosomal contacts. J. Rabbit Polyclonal to FCGR2A Log2 contact enrichment of A-A and B-B contacts for inter-chromosomal contacts, K. Percentage of genome covered by TADs called by HOMER v4.7. L. Quantity of TADs called by HOMER v4.7. M. Quantity of loops called from the algorithm of Juicer tools v0.7.5. N. Average standardized AMG 337 insulation score at the related G1 control TAD boundaries (hires or r1, r2 average) called by HOMER v4.7 in the respective conditions. O. Average standardized insulation score in the TAD boundaries called by HOMER v4.7 in the respective conditions. P. Quantity of loops called from the algorithm of Juicer tools v0.7.5; please note that the number of loops that can be called depends on the number of unique read pairs. This needs to be taken into consideration when comparing corner peaks between different experiments. elife-52091-supp1.xlsx (34K) GUID:?ED6CCC31-86F0-4AB7-B7D6-0A7C531F5636 Supplementary file 2: Quantity of cells analyzed by FISH and statistical significance. Quantity of cells analyzed by FISH in Number 5figure product 2 for?control,CTCF, SCC1, STAG1, STAG2 and two times STAG1/STAG2 RNAi. Statistical significance is definitely measured by t-test relative to the control. elife-52091-supp2.xlsx (15K) GUID:?9A254B42-E218-407B-B329-FA4BA1A964D2 Transparent reporting form. elife-52091-transrepform.docx (246K) GUID:?192E80DD-048C-4C84-86B1-AE89756B688C Data Availability StatementSequencing data have been deposited in GEO less than accession code “type”:”entrez-geo”,”attrs”:”text”:”GSE138405″,”term_id”:”138405″GSE138405, and is available at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=”type”:”entrez-geo”,”attrs”:”text”:”GSE138405″,”term_id”:”138405″GSE138405. The following dataset was generated: Wutz G, AMG 337 Ladurner R, St Hilaire B, Stocsits R, Nagasaka K, Pignard B, Sanborn A, Tang W, Vrnai C, Ivanov M, Schoenfelder S, vehicle der Lelij P, Huang X, Drnberger G, Roitinger E, Mechtler K, Davidson IF, Fraser P, Aiden EL, Peters JM. 2020. ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL. NCBI Gene Manifestation Omnibus. GSE138405 The following previously published dataset was used: Gordana Wutz, Roman R Stocsits. 2017. Topologically associating chromatin and domains loops rely on cohesin and so are governed by CTCF, WAPL and PDS5 protein. NCBI Gene Appearance Omnibus. GSE102884 Abstract Eukaryotic genomes are folded into loops. It really is thought these are shaped by cohesin complexes extrusion, either until loop enlargement is imprisoned by CTCF or until cohesin is certainly taken off DNA by WAPL. Although.